Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (29): 5428-5432.doi: 10.3969/j.issn. 2095-4344.2012.29. 022

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Characterization and biocompatibility of silk fibroin/hyaluronic acid composite porous scaffolds

Fan Zhi-hai1, Lu Fu-nan2, Zhang Feng3, Shen Yi-xin1   

  1. 1Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China;
    2Department of Orthopedics, People’s Hospital of Luzhi, Suzhou 215127, Jiangsu Province, China;
    3School of Basic Medicine and Biology, Soochow University, Suzhou 215123, Jiangsu Province, China
  • Received:2012-04-09 Revised:2012-04-21 Online:2012-07-15 Published:2012-07-15
  • Contact: Shen Yi-xin, Professor, Master’s supervisor, Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China shenyx@ mail.szldbz.gov.cn
  • About author:Fan Zhi-hai☆, Studying for doctorate, Attending physician, Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China fanzh2006@163.com

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Abstract:

BACKGROUND: Silk fibroin and hyaluronic acid with good biocompatibility have the features of protein and glycosaminoglycan in the extracellular matrixs.
OBJECTIVE: To prepare silk fibroin/hyaluronic acid composite scaffolds using lyophilization, and to evaluate their characterization and cellular biocompatibility.
METHODS: The silk fibroin/hyaluronic acid composite scaffold was prepared by lyophilization at a ratio of 10:1 and characterized by scanning electron microscope and Fourier Transform infrared spectroscopy. Passages 3-5 bone marrow mesenchymal stem cells from rats at a density of 1×108 cells/L were seeded on the silk fibroin/hyaluronic acid composite scaffold to observe cellular biocompatibility by scanning electron microscope and Laser confocal microscope.
RESULTS AND CONCLUSION: The silk fibroin/hyaluronic acid composite scaffold was successfully prepared by lyophilization, showing better porous three-dimensional structure compared with pure silk fibroin scaffold. Good cell adhesion, spreading and proliferation behavior on the silk fibroin/hyaluronic acid composite scaffolds were observed, indicating that the cellular biocompatibility of the composite scaffolds is preliminarily confirmed.

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